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Water Microbiology

Water Microbiology

Alyse Rose Microbiology Lab Bacterial Unknown March 25, 2013 Bacterial Unknown Report Each student was given an unknown bacteria to figure out. I was given the unknown bacteria S38. Everybody is supposed to do all sorts of test to identify the bacteria. The first thing I did was smear my bacteria on a liquid medium. I then proceeded to incubate the medium for 24-48 hours. 1. GRAM STAIN The next step I took in finding my unknown bacteria was to gram stain it.This is used to differentiate the bacteria. The different staining reagents are: crystal violet, grams iodine, acetone-alcohol, and grams safranin. Under the microscope it was a pink color, which means Gram Negative. Also, the shape was a rod. 2. KLIGER’S IRON AGAR (KIA) The next step I did was the KIA slant. This is for the Negative bacteria to see if they are in the Enteriobacteriaceae group. There are many different results than can occur, like: 1.Yellow slant/yellow deep- (A/A) glucose & lactose are fermented; if the butt has bubbles in it or is displaced, the tube is gas positive. 2.

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